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Journal: PeerJ
Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting
doi: 10.7717/peerj.20583
Figure Lengend Snippet: (A) Green fluorescent protein detection in LN229 and U87MG cell lines. (B) The detection of expression level of ASIC2 by shRNA plasmid. (C) Detection of ASIC2 expression level by shRNA plasmid. (D) Detection of ASIC2 expression level by overexpression plasmid. (E) Detection of cell proliferation ability with U87MG cells. (F) Detection of cell proliferation ability of LN229 cells in each group. (G) Detection of cell clone forming ability of U87MG and LN229 cells. (H) Statistical analysis of colony formation in U87MG and LN229 cells. (I) Detection of cell invasion ability of U87MG and LN229 cells. (J) Statistical analysis of the number of U87MG and LN229 cells migrating through the chamber. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01 vs control, # P < 0.05, ## P < 0.01 vs plasmid control.
Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the
Techniques: Expressing, shRNA, Plasmid Preparation, Over Expression, Control
Journal: PeerJ
Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting
doi: 10.7717/peerj.20583
Figure Lengend Snippet: (A) Detection of cell migration ability of glioma groups. (B) Statistical analysis of the wound closure rate in U87MG cells. (C) Statistical analysis of the wound closure rate in LN229 cells. (D–E) Regulation of cell cycle by ASIC2 on LN229 cells. (F, H) ASIC2 regulates the expression levels of P21, cycline D1 proteins in LN229. (G, I) Statistical analysis of protein expression in LN229 cells after ASIC2 interference and overexpression. (J–K) ASIC2 regulates the expression levels of MMP2, calcineurin and NFAT1 proteins in glioma cells. (L–O) Statistical analysis of protein expression in U87MG and LN229 cells after ASIC2 interference and overexpression. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control, # P < 0.05, ## P < 0.01, ### P < 0.001 vs plasmid control.
Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the
Techniques: Migration, Expressing, Over Expression, Control, Plasmid Preparation